BsiWI from New England Biolabs

Product Specs
ItemBsiWI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0553S
Concentration12 u/µL
Quantity300 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/GTACG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Broad-spectrum humanized monoclonal neutralizing antibody against SARS-CoV-2 variants, including the Omicron variantFrontiers in Cellular and Infection MicrobiologyAugust 14, 2023Kun Wen, Jian-Piao Cai, Xiaodi Fan, Xiaojuan Zhang, Cuiting Luo, Kai-Ming Tang, Huiping Shuai, Lin-Lei Chen, Ricky Ruiqi Zhang, Jianwen Situ, et al.R0652L and R3131L) for the heavy chain, and AfeI and BsiWI (New England Biolabs, Ipswich, USA; Cat# R0553L) for the light chain. Equal amounts of heavy chain and light chain plasmids were transfected into Expi293F

(2) Adipose Tissue-Derived Extracellular Vesicles Contribute to Phenotypic Plasticity of Prostate Cancer CellsInternational Journal of Molecular SciencesJanuary 8, 2023Allison Mathiesen, Bronson Haynes, Ryan Huyck, Michael Brown, Anca Dobrian(Zymoresearch, cat # D4213). Plasmids were linearized using BsiWI restriction enzyme (New England BioLabs, cat # R0553S). PC3-ML were transfected with all three plasmids using NucleofectorTM (Lonza, Cat # VPB-1003). Selection

(3) Genetic evidence that uptake of the fluorescent analog 2NBDG occurs independently of known glucose transportersPLOS ONEAugust 24, 2022Lucas J. D’Souza, Stephen H. Wright, Deepta Bhattacharyaconstructs with already cloned gRNA sequences and control lentiGuide-puro with BsiWI-HF and MluI-HF (R0553 and R3198, NEB) to exclude the puromycin N-acetyltransferase gene. Sequences encoding fluorescent proteins

(4) AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decayMolecular cellAugust 4, 2022Cho H, Abshire ET, Popp MW, Pröschel C, Schwartz JL, Yeo GW, Maquat LE.Polymerase,Thermo Fisher Scientific,N8080160 ApaI,NEB,R0114S KpnI-HF,NEB,R3142S XhoI,NEB,R0146S BsiWI,NEB,R0553L SspI,NEB,R0132S Quick CIP,NEB,M0525S T4 DNA Ligase,Takara Bio,2011B "T4 DNA Polymerase, LIC-qualified",Novagen,70099 Circligase

(5) Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenomeNature Cell BiologyJuly 4, 2022Yingying Meng et al.LL) with 2 U (or 4 U when indicated) of HhaI (NEB, R0139S) restriction enzyme at 37 °C, Bsiwl (NEB, R0553S) at 55 °C or with TaiI (Thermo, ER1141) at 65 °C for 12 h. After incubation, reactions were subjected

(6) Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?Frontiers in immunologyJanuary 1, 2021Ehlers AM, den Hartog Jager CF, Kardol-Hoefnagel T, Katsburg MMD, Knulst AC, Otten HG.restriction enzyme, R0131L, New England BioLabs, Ipswich, Massachusetts, USBsiWI restriction enzyme, R0553L, New England BioLabs, Ipswich, Massachusetts, USAvrII restriction enzyme, R0174L, New England BioLabs,

(7) Integrative analysis suggests cell type-specific decoding of NF-κB dynamicsScience signalingFebruary 25, 2020Martin EW, Pacholewska A, Patel H, Dashora H, Sung MH.plasmid were digested with restriction enzymes from NEB (EcoR1-HF, R3101S; EcoRV-HF, R3195S; BsiW1, R0553S; BsrGI-HF, R3575S), where applicable. The plasmid was dephosphorylated with Antarctic Phosphatase (NEB;

(8) LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma CellsImmunityJuly 17, 2018Lino AC, Dang VD, Lampropoulou V, Welle A, Joedicke J, Pohar J, Simon Q, Thalmensi J, Baures A, Flühler V, et al.Mix,Eurogentec,Cat. #NU-0010-100 AgeI–HF,NEB,Cat. #R3552L SalI–HF,NEB,Cat. #R3138L BsiWI,NEB,Cat. #R0553L NEBuffer 1,NEB,Cat. #B7001S Gel and PCR clean up kit,Macherey-Nagel,Cat. #740609.250 10X Buffer T4

(9) Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?NanotheranosticsJanuary 1, 2018Paramonov VM, Desai D, Kettiger H, Mamaeva V, Rosenholm JM, Sahlgren C, Rivero-Müller A.generated pcDNA 3.1/V5-His TOPO-TA plasmid by means of double-digest with BsiWI (New England Biolabs, Cat# R0553) and BssHII (Promega, Cat# R6831) and gel-purified. The derived isolate was further directionally cloned

(10) Protein and Antibody Engineering by Phage DisplayMethods in EnzymologySeptember 3, 2016J.C. Frei, J.R. LaiM0531S) Restriction enzymes: BssHII, BsiWI, NheI, NsiI, FseI (NEB, Ipswich, MA, catalog #s: R0199S, R0553S, R0131S, R0127S, R0558S, respectively) Alkaline Phosphatase, Calf Intestinal (NEB, Ipswich, MA, catalog

(11) A new class of highly potent, broadly neutralizing antibodies isolated from viremic patients infected with dengue virusNature ImmunologyFebruary 1, 2015Wanwisa Dejnirattisai et al.a gift from E. Harris. Restriction enzymes, Agel-HF (R3552), SalI-HF (R3138), XhoI (R0146), BsiWI (R0553), calf-intestinal ALP (M0290) and T4 DNA ligase (M0202L) were from NEB. Bovine serum albumin (BSA, A7030),

(12) Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigenNature protocolsJanuary 1, 2009Smith K, Garman L, Wrammert J, Zheng NY, Capra JD, Ahmed R, Wilson PC.(New England Biolabs Inc., http://www.neb.com, R0552L) BsiWI (New England Biolabs Inc., http://www.neb.com, R0553L) SalI (New England Biolabs Inc., http://www.neb.com, R0138L) XhoI (New England Biolabs Inc., http://www.neb.com,

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BsiWI

BsiWI from New England Biolabs

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BsiWI from New England Biolabs

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